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Antioxidant Activity of Ficusp seudopalma Blanco and its Cytotoxic Effect on Hepatocellular Carcinoma and Peripheral Blood Mononuclear Cells

Librado A. Santiago, Paolo Robert P. Bueno, Clarissa Bel M. Buno, Deza Lyn M. Santos

Abstract


Cancer is characterized by the rapid and uncontrolled propagation of tumor cells caused by oxidative stress, contributing to
mutations in the DNA. Plant antioxidants bind toxic free radicals and inhibit further cellular damage. Due to limited
information on the pharmacologic activity of Ficuspseudopalma, an endemic Philippine medicinal plant, this study was
performed to evaluate its antioxidant activity in vitro and to investigate its effect on cell inhibition of Hepatocellular Carcinoma
(HepG2) cells and Peripheral Blood Mononuclear Cells (PBMC). Ethanolic extraction yielded 7.04% crude extract. The Total
Phenolic Content and Total Flavonoid Content were 360.83 ppm and 309.26 ppm, respectively. Different concentrations of
ethanolic crude extract exhibited concentration‐dependent response on DPPH (IC50=331.76 μg/mL), nitric oxide (IC50=19.81
μg/mL) and FRAP (IC50>53.04 μg/mL). Cytotoxicity of the ethanolic extract on HepG2 yielded an IC50at 300 μg/mL and had a
concentration‐ and time‐ dependent inhibition response. In contrast, F. pseudopalma showed concentration‐dependent growth
stimulation of PBMC. These findings suggest that the ethanolic crude extract of F. pseudopalmacontains bioactive ingredients
that exhibit antioxidant and cytotoxic activity that may help liver cancer patients. Furthermore, the extract is not toxic to
normal cells.

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